Assess sample quantity for experimental applications with protein quantitation, DNA quantitation and reporter activity assays, and spectrophotometry instrumentation.
All assays are easy to use, require little reagent preparation, and give accurate and reproducible results. * To adapt the RC DC assay to a microplate format, follow the micro test tube (microfuge tube) assay protocol in the RC DC instruction manual up to the centrifugation step where the supernatant is discarded. The pellet can then be transferred to the microplate, and the microplate assay protocol in the DC protein assay manual can be followed. In a typical protein assay, a chemical reagent is added to a protein sample, producing a visible result, such as a color change in the sample solution. This color change is quantitated with a spectrophotometer or microplate reader, and compared to a standard curve of known concentrations of protein versus their absorbance after reaction with the reagent. The amount of protein in the unknown sample is determined by interpolation, reading the concentration of protein on the standard curve that corresponds to its absorbance.
The SmartSpec™ Plus spectrophotometer is a benchtop UV/visible spectrophotometer with a wide range of quantitation applications. Also offered is a line of compatible quartz and UV-transparent plastic cuvettes.
DNA quantitation, β-galactosidase, and β-glucuronidase assay kits and cuvettes, as well as a broad range of filters, are available for use with the VersaFluor™ fluorometer, which is now discontinued.
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