Three CHEF systems that incorporate different technologies for optimal resolution in various size ranges, such as PACE, FIGE, and AFIGE are offered. Agarose, reagents, standards, and kits for PFGE are available.
The multi-state mode of the CHEF Mapper system enhances resolution in selected fragment size ranges. Each vector (angle of pulse) can be assigned its own voltage (field intensity) and its own switch time (duration of pulse). Up to eight different states may be combined into one run to optimize the separation of subsets of fragments in the sample on the left. The application of secondary pulses of defined voltage, duration, angle and frequency can enhance the separation and resolution of very large DNA molecules in the image on the right. These secondary pulses may release DNA caught in the gel matrix.
Mobility effects of nonlinear switch-time ramps on the CHEF Mapper� system. Molecular size vs. migration for linear, concave, and convex ramps. The convex ramp results in the widest linear range.
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