Bio-Rad offers four colorimetric protein assay kits for the measurement of protein levels using modifications of the Bradford and Lowry assay method. Disposable standard and semimicro plastic cuvettes ideal for these protein assays are also available.
* Nonidet P-40 >0.25%, Triton C-100 >0.05%, Tween 20 >0.01%, SDS >0.025% ** DTT >1 mM, EDTA >1 mM
All assays are easy to use, require little reagent preparation, and give accurate and reproducible results. * To adapt the RC DC assay to a microplate format, follow the micro test tube (microfuge tube) assay protocol in the RC DC instruction manual up to the centrifugation step where the supernatant is discarded. The pellet can then be transferred to the microplate, and the microplate assay protocol in the DC protein assay manual can be followed. In a typical protein assay, a chemical reagent is added to a protein sample, producing a visible result, such as a color change in the sample solution. This color change is quantitated with a spectrophotometer or microplate reader, and compared to a standard curve of known concentrations of protein versus their absorbance after reaction with the reagent. The amount of protein in the unknown sample is determined by interpolation, reading the concentration of protein on the standard curve that corresponds to its absorbance.
The DC (detergent compatible) protein assay uses a colorimetric method to determine protein concentration following detergent solubilization. The reaction is similar to the well-documented Lowry assay.
The Quick Start Bradford assay kit is a simple one step procedure. with no need to dilute standards for determining the concentration of protein in a solution.
The RC DC (reducing agent and detergent compatible) protein assay is a colorimetric assay for protein determination in the presence of reducing agents and detergents.
Standard 3.5 ml or semimicrovolume 1.5 ml polystyrene cuvettes that are ideal for protein assays.
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