The Profinity eXact fusion-tag system is an E. coli–based expression and purification system that generates a tag-free protein containing its native N-terminal amino acid sequence in a single step.
SDS-PAGE analysis of a purification of Profinity eXact fusions to a 26 kD green fluorescent protein (GFP) and to a 40 kD maltose binding protein (MBP). Crude E. coli lysate containing the expressed proteins (2 ml) was loaded onto a Bio-Scale Mini Profinity eXact 1 ml cartridge on a BioLogic DuoFlow™ system with bind/wash buffer at 1 ml/min. The cartridge was washed with 10 column volumes (CV) of the same buffer at 1 ml/min. Proteins were eluted with 3 CV sodium fluoride buffer at room temperature and at 0.1 ml/min for 30 min. Total purification time required to generate the tag-free proteins without addition of protease was approximately 60 min.
Call us at 1-800-268-0213
Please reenter your email address in the correct format.
Please enter your email address.
Your subscription information already exists, we will send you an email with specific instructions to manage your existing subscription profile.
To receive the latest news, promotions, and more, sign up for Bio-Rad updates by entering your email address below. You can elect to receive only the types of Bio-Rad communications that are of interest to you..
Copyright © 2014 Bio-Rad Laboratories, Inc. All rights reserved.