Discover how Bio-Rad’s innovative digital PCR products provide absolute quantification of target DNA or RNA molecules without the use of standard curves. Droplet Digital™ PCR (ddPCR™), Bio-Rad’s unique digital PCR technology, has already led to several research breakthroughs.
Browse the growing list of cited peer-reviewed journal articles in Droplet Digital™ PCR research featuring Bio-Rad products.
The publications appear in journals such as PLOS ONE, Cell, Nucleic Acids Research, and Nature Methods and span the following applications:
Success Stories: Articles
Genetic Variation Analysis: Single Nucleotide Polymorphism: Validation of Allele-Specific Expression Predicted by RNA-Seq in Human Brain Specimens In the context of human brain development, the QX100™ Droplet Digital™ PCR System yields unambiguous results for genotype identification of low-expression genes and provides absolute quantification of allele-specific expression (ASE) without the use of a standard. Download the article.
Genetic Variation Analysis: Copy Number Variation: DUF1220 Copy Number Variation across Healthy and Diseased Populations Studying copy number variation (CNV) with the QX100 Droplet Digital PCR System allows the robust identification of genetic factors such as DUF1220 in neurogenetic diseases between individuals with greater accuracy and precision, while effectively validating array CGH data. Download the article.
Genetic Variation Analysis: Single Nucleotide Polymorphism: Detection of Single Nucleotide Variation Frequencies by Droplet Digital™ PCR The QX100 Droplet Digital PCR System validated DNA sequencing results of induced pluripotent stem cells (iPSCs). In addition to confirming the high frequency of single nucleotide variations (SNVs), a de novo type of SNV was identified. Download the article.
Cancer Mutation Analysis: How Droplet Digital PCR Has Revolutionized Noninvasive Plasma-Based Detection of Mutations Performing Droplet Digital PCR with the QX100 Instrument effectively detected and accurately quantified the EGFR T790M marker in targeted therapies with limited cancer tumor samples. Download the article.
Viral Quantification: Adeno-Associated Virus Vector Genome Titer Assay Determining the adeno-associated virus (AAV) vector genome (vg) titer assay is critical in gene therapy applications. The improved sensitivity observed with Droplet Digital PCR (ddPCR™) technology makes it highly suitable for titration of clinical-grade AAV vector preparations. Download the article.
Gene Expression: Digital Quantification of Potential Therapeutic Target RNAs Measuring the exact number of long noncoding RNAs (lncRNAs) in subcellular compartments with Droplet Digital PCR (ddPCR) provides insights into their role and mechanism of action. Download the article.
Third Generation PCR: A Novel Way to Accurately Measure Copy Number Variations in Human Genome The McCarroll Lab uses Droplet Digital technology to study the distribution and molecular properties of the genome and its influence on gene expression, as well as the genetics underlying schizophrenia and bipolar disorder. Download the article.
Success Stories: Videos
Droplet Digital™ PCR opens new perspectives in HIV research. Sangamo BioSciences, Inc. is developing HIV therapeutics toward a functional cure using the company’s proprietary zinc finger DNA-binding protein technology. Download the article.
Professor Alec Morley, a pioneer in digital PCR, and his colleagues in Adelaide have developed tests for detecting acute lymphoblastic leukemia (ALL) and chronic myeloid leukemia (CML) using the unrivaled sensitivity of the QX200 Droplet Digital PCR System.
This comprehensive digital PCR resource guide includes detailed information about:
Digital PCR Application Notes
Droplet Digital PCR: Ultra-Sensitive Validation Technology for RNA-Seq Droplet Digital PCR (ddPCR™) is a digital technology that counts individual molecules with high precision and linearity over a five-log range. It can be used for validating RNA-Seq reads with high accuracy while eliminating distortion of sample composition and loss of sensitivity due to sampling error. Download the article.
Droplet Digital PCR: Ultra-Sensitive Rare Event Detection Using Bio-Rad’s QX100™ Droplet Digital™ PCR System Partitioning samples into droplets, a step of the Droplet Digital PCR (ddPCR) workflow, increases the relative representation of rare targets and enables the precise quantification of rare events — such as rare mutation detection (RMD) and rare sequence detection (RSD) — on the QX100 Droplet Digital PCR System. Download the article.
Droplet Digital PCR: Linking Microbial Physiology and Phylogeny Using Bio-Rad’s QX100 Droplet Digital PCR System Droplet Digital PCR (ddPCR) technology demonstrates the association of proteorhodopsin and SAR11 16S rRNA signatures while bypassing extensive metagenomic analysis.
The QX100 Droplet Digital PCR instrument enables users to investigate microbial diversity and metabolic interactions while uncovering connections between physiology and phylogeny. Download the article.
Droplet Digital PCR: High-Resolution Copy Number Variation Analysis Analyses of copy number (CN) variation with the QX100 Droplet Digital PCR System yield high-resolution findings with a precision exceeding that of any traditional technologies.
This low-cost, high-throughput method can reliably validate CN alterations identified by next-generation sequencing (NGS). Download the article.
QX100 Droplet Digital PCR System for Accurate Quantification of Illumina TruSeq Libraries Creating and combining balanced sequencing libraries requires the accurate quantification of the library load prior to sequencing using next-generation sequencing (NGS) technologies.
Here, the QX100 Droplet Digital PCR System was incorporated into the Illumina TruSeq library preparation workflow and enabled consistent library loading and efficient utilization of Illumina TruSeq libraries. Download the article.
Probing Copy Number Variations Using Bio-Rad's QX100 Droplet Digital PCR System The QX100 Droplet Digital PCR System provides excellent discrimination of the copy number states of clinically important loci, even for small fold differences. The consistently accurate and precise results do not require a large number of replicates and are much more tolerant to factors that affect real-time PCR (qPCR). Download the article.
Bio-Rad’s second-generation digital PCR system provides absolute quantification of target DNA or RNA molecules using EvaGreen or TaqMan Hydrolysis Probes. Unmatched sensitivity and precision for various applications.
Bio-Rad's first-generation digital PCR system provides absolute quantification of target DNA molecules using probe-based digital PCR applications.
PrimePCR™ assays for Droplet Digital™ PCR (ddPCR™) enable precise quantification without a standard curve. Digital PCR assays are guaranteed for performance and are the only content validated assays for digital PCR.
Bio-Rad's Droplet Digital PCR Supermixes are ready-to-use universal reaction mixes that have been optimized to deliver maximum PCR efficiency, specificity, and sensitivity in ddPCR.
Bio-Rad's digital PCR kits allow accurate quantification of DNA libraries before next-generation sequencing (NGS) on Illumina RNA-Seq and Ion Torrent AmpliSeq Platforms.
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