Activated media for spontaneous ligand immobilization allow for the creation of an affinity matrix of choice by coupling a ligand, such as an antibody, enzyme, or antigen.
* Refer to bulletin 3193 for purification conditions. ** For example, Profinity GST resin is available only in prepacked cartridges. ***Profinity eXact Purification Resin.
Affi-Gel 10 and Affi-Gel 15 are complementary affinity media that offer rapid, high-efficiency coupling for all ligands that have a primary amino group — including proteins with any isoelectric point (pl) and low molecular weights ligands. Both Affi-Gel 10 and 15 are N-hydroxysuccinimide esters of a derivatized crosslinked agarose gel bead support, and both couple to ligands spontaneously in aqueous or nonaqueous solution.
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