Following electrophoresis or immunodetection, gels and blots can be imaged and analyzed using a variety of imaging systems. The signals are digitized by dedicated image acquisition devices, data is analyzed with sophisticated software and then stored for later use.This section provides an overview of the suite of gel and blot imaging systems that Bio-Rad offers, highlights their features, and discusses their respective applications.
Related Topics: Image Analysis Software.
Densitometers are based on high-performance document scanners utilizing visible light for analysis of electrophoresis gels and blots stained with visible dyes. Bio-Rad's GS-900™ Calibrated Densitometer can be used to image both gels and blots and has a linear response up to 3.0 optical units in gray levels.
GS-900 Calibrated Densitometer
Charge-coupled device (CCD) cameras operate with either transillumination, provided by UV or visible light boxes positioned underneath the gel or blot for imaging a variety of stains (Coomassie, silver, fluorescence), or epi-illumination, which detects blots using colorimetric or fluorescent techniques. Supercooled CCD cameras reduce image noise, allowing detection of faint luminescent signals.
The ability to visualize gels without a staining step has gained popularity recently. Stain-free technology has several advantages over staining procedures. In particular, it eliminates the need for a lengthy destaining step, makes visualization faster and more efficient, and eliminates disposal of organic waste generated during destaining. The stain-free enabled imagers come with an automated imaging and analysis system and give less background compared to traditional Coomassie staining procedures, thereby reducing variability between and within gels.
See how you can streamline your protein electrophoresis and western blotting experiments.
See the Imaging Systems Selection Guide below for full details of the capabilities of each imaging system.
* Optimal with low fluorescence PVDF membrane.
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