Protein Solubilization

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en-ca LUSP3KFD4 Protein Solubilization Protein Solubilization /webroot/web/html/lsr/solutions/technologies/protein_electrophoresis <p>Protein solubilization is the process of breaking interactions involved in protein aggregation, which include disulfide bonds, hydrogen bonds, van der Waals forces, ionic interactions, and hydrophobic interactions. If these interactions are not prevented, proteins can aggregate or precipitate, resulting in artifacts or sample loss. For successful electrophoretic separation, proteins must be well solubilized. This section describes various agents used for protein solubilization, their method of action, and their compatibility to different types of samples. It also provides troubleshooting tips.</p> <p>Cell lysis and protein solubilization are ideally carried out in the sample buffer that is recommended for the particular electrophoresis technique, especially when native electrophoresis is the method of choice. If this is not possible, proteins must be prepared in sample solubilization solutions that typically contain a number of compounds, including chaotropic agents, detergents, reducing agents, buffers, salts, and ampholytes. These are chosen from a list of compounds that meet the requirements, both electrically and chemically, for compatibility with the electrophoretic technique being used. In these cases, the sample will have to be diluted with concentrated electrophoresis sample buffer to yield a 1x final buffer concentration. Sample solubilization methods for 2-D electrophoresis applications are discussed in the <a href="/en-us/applications-technologies/2-d-electrophoresis?ID=LUSQF04EH">2-D Electrophoresis</a> section.</p> <p><strong>Related Sections</strong>: <a href="/en-us/applications-technologies/cell-disruption?ID=LUSP2D1FX">Cell Disruption</a>, <a href="/en-us/applications-technologies/removal-interfering-substances?ID=LUSP4LJSZ">Removal of Interfering Substances</a>, and <a href="/en-us/applications-technologies/protein-electrophoresis-sample-preparation/sample-quantitation?ID=LUSP5JKAJ">Sample Quantitation</a>.</p> Detergents <p>Detergents are classified as nonionic, zwitterionic, anionic, and cationic, and they disrupt hydrophobic interactions between and within proteins. Some proteins, especially membrane proteins, require detergents for solubilization during isolation as well as to maintain solubility. Nonionic detergents such as NP-40 and Triton X-100 are not very effective in solubilizing hydrophobic proteins; zwitterionic detergents such as CHAPS and sulfobetaines (for example, SB 3&ndash;10 or ASB 14) provide higher solubilization efficiency, especially for integral membrane proteins (Luche et al, 2003). Sample preparation for SDS-PAGE commonly uses the anionic detergent sodium dodecyl sulfate (SDS), which is unparalleled in its ability to efficiently and rapidly solubilize proteins.</p> <div class="top"><a href="#helptop">Back to Top</a></div> Reducing Agents <p>Thiol reducing agents such as 2-mercaptoethanol &beta;ME and dithiothreitol (DTT) disrupt intramolecular and intermolecular disulfide bonds and are used to achieve complete protein unfolding and to maintain proteins in their fully reduced states (see figure below).</p> <p>&beta;ME is volatile, evaporates from solutions, and reduces protein disulfide bonds by disulfide exchange. There is equilibrium between free thiols and disulfides, so &beta;ME is used in large excess in sample buffers to drive the equilibrium reaction toward completion. If the concentration of &beta;ME drops and proteins reoxidize, they may yield fuzzy or spurious artifactual bands upon gel electrophoresis of the sample.</p> <p>DTT is less volatile and is altered during the disulfide reduction reaction to form a ring structure form its original straight chain. The equilibrium favors protein reduction, so lower concentrations of DTT are needed (higher concentrations are recommended for proteins with large numbers of disulfide bonds).</p> <p>Phosphines such as tributylphosphine (TBP) and triscarboxyethylphosphine (TCEP)* offer an alternative to thiols as reducing agents because they can be used at lower concentration and over a wider pH range than the sulfhydryl reductants.</p> <p><img src="/webroot/web/images/lsr/solutions/technologies/protein_electrophoresis_blotting_and_imaging/protein_electrophoresis/technology_detail/pet132_img1.jpg" alt="" width="245" height="264" /></p> <p class="caption"><strong>Reduction of proteins with DTT.</strong></p> <p><em>*TCEP is included in Bio-Rad&rsquo;s XT sample buffers. Though TCEP can be added to SDS-PAGE sample buffer, it must first be neutralized with NaOH; otherwise it will hydrolyze proteins.</em></p> <div class="top"><a href="#helptop">Back to Top</a></div> Chaotropic Agents <p>Chaotropic compounds such as urea disrupt hydrogen bonds and hydrophobic interactions both between and within proteins. When used at high concentrations, they destroy secondary protein structure and bring otherwise-insoluable proteins into solution.</p> <p>Urea and substituted urea such as thiourea improve solubilization of hydrophobic proteins.</p> <p>Samples containing urea and thiourea can be used in SDS-PAGE when diluted with SDS-PAGE sample buffer. In this case, the protein solution may not be heated above 37&deg;C, since urea and thiourea can hydrolyze to cyanate and thiocyanate, respectively, and modify amino groups on proteins (carbamylation), giving rise to artifactual charge heterogeneity.</p> <div class="top"><a href="#helptop">Back to Top</a></div> Buffers and Salts <p>Both pH and ionic strength influence protein solubility; therefore, buffer choice is important, especially when native electrophoresis conditions are required. Many proteins are more soluble at higher pH, so Tris base is often included to elevate the pH. However, proteins differ in their solubility at different pH values, so the use of different buffers can extract different sets of proteins. The choice of buffer and pH of the sample preparation solution can strongly influence which proteins are successfully solubilized.</p> <p>Even in the presence of detergents, some proteins have stringent salt requirements to maintain their solubility, but salt should be present only if it is an absolute requirement. Excess salt in SDS-PAGE samples causes fuzzy bands and narrowing of gel lanes toward the bottom of the gel. If the ionic strength is very high, no visible bands will appear in the lower part of the gel (a vertical streak will appear instead) and the dye front will be wavy instead of straight.</p> <div class="top"><a href="#helptop">Back to Top</a></div> <p>Ideally, cell lysis and protein solubilization are carried out in the sample buffer that is recommended for the particular electrophoresis technique, especially for native electrophoresis. If this is not possible, dilute the protein solution with concentrated electrophoresis sample buffer to yield a 1x final buffer concentration.</p> <p><strong>ReadyPrep&trade; protein extraction kits</strong></p> <p>Bio-Rad offers a number of ready-to-use kits and solutions for protien extraction. These kits and reagents facilitate the solubilization of the sample and preparation for 2D electrophoresis separation:</p> <ul> <li><a href="/en-us/product/readyprep-protein-extraction-kit-total-protein?ID=749eaa83-8fb4-4854-bc8a-84ab01195fb9">ReadyPrep extraction kit (total protein)</a></li> <li><a href="/en-us/sku/1632102-readyprep-sequential-extraction-kit-reagent-1?ID=1632102">ReadyPrep sequential protein extraction reagent</a></li> <li><a href="/en-us/sku/1632085-readyprep-protein-extraction-kit-soluble-insoluble">ReadyPrep sequential protein extraction (soluble/insoluble)</a></li> </ul> <div class="top"><a href="#helptop">Back to Top</a></div> Troubleshooting <table class="pd_table pd_gridlines" border="0"> <tbody> <tr class="pd_tableheader pd"> <td width="26%"><strong>Problem</strong></td> <td width="26%"><strong>Cause</strong></td> <td><strong>Solution</strong></td> </tr> <tr> <td valign="top">Laemmli sample buffer turns yellow</td> <td valign="top">Sample buffer is too acidic</td> <td valign="top">Add Tris base until buffer turns<br /> blue again</td> </tr> <tr> <td valign="top">Sample very viscous</td> <td valign="top">High DNA or carbohydrate content</td> <td valign="top"> <p>Fragment DNA with ultrasonic waves during cell lysis and protein solubilization</p> <p>Add endonucleases like Benzonase</p> <p>Precipitate protein with TCA/acetone (<a href="/en-us/product/readyprep-2-d-cleanup-kit?ID=bbf3a0be-188f-45c7-b81e-e9706ec77c02">ReadyPrep 2-D cleanup kit</a>) to diminish carbohydrate content</p> </td> </tr> </tbody> </table> <div class="top"><a href="#helptop">Back to Top</a></div> References <p>Luche S et al. (2003). Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis. Proteomics 3, 249&ndash;253.</p> <div class="top"><a href="#helptop">Back to Top</a></div> Protocols <table id="carttablealigned" class="literature_table" style="height: auto; width: 583px;" border="0" cellspacing="0" cellpadding="0"> <tbody> <tr></tr> <tr> <td width="100">6194</td> <td width="350">Protein Sample Generation General Tips</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6194.pdf" target="_blank" rel="noopener noreferrer"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6195</td> <td width="350">Protein Sample Preparation (Human Tissue)</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6195.pdf" target="_blank" rel="noopener noreferrer"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6196</td> <td width="350">Protein Sample Preparation (Mammalian Tissue)</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6196.pdf" target="_blank" rel="noopener noreferrer"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6197</td> <td width="350">Protein Sample Preparation (Plant Leaves)</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6197.pdf" target="_blank" rel="noopener noreferrer"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6198</td> <td width="350">Protein Sample Preparation (Microbial Cultures)</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6198.pdf" target="_blank" rel="noopener noreferrer"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6199</td> <td width="350">Buffer Formulations</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6199.pdf" target="_blank" rel="noopener noreferrer"><span>Click to download</span></a></td> </tr> </tbody> </table> 3096 templatedata/internet/documentation/data/LSR/Literature/3096_0910140925966.xml Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B 3096 /webroot/web/pdf/lsr/literature/Bulletin_3096.pdf Literature PDF Brochures_and_Specifications /webroot/web/images/general/icons/icon_pdf.gif Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure No Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B 3096 bio-spin, dynamic range reduction, sample prep, preparation, prep cell, rotofor, enrichment of low-abundance, isoelectric focusing, lit3096, membrane i, micro bio spin columns, model 491, proteins, truview cuvettes, cell lysis, expression proteomics, mini, prep, proteominer kits, quick start bradford assay, ready prep, readyprep, 2-d electrophoresis, affi-gel blue, albumin, aurum, chemical reagents, ief, microrotofor, protean ii, rc dc, smartspec plus, spectrophotometer, 165-2435, 1652435 Life Science Research/Products/Protein Sample Preparation/Protein Extraction/MicroRotofor Cell Lysis Kits ->MT::5e97c5be-3707-4c08-ae55-25e883f2ab99##Life Science Research/Products/Protein Sample Preparation/Protein Extraction/ReadyPrep Protein Extraction Kit (Total Protein) ->MT::749eaa83-8fb4-4854-bc8a-84ab01195fb9##Life Science Research/Products/Sample Quantitation/Protein Assay Kits and Cuvettes/<em>RC DC</em> Protein Assay ->MT::f58fdf1d-5d0b-4f31-93e2-f812bd7e7eab##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Electrophoresis Buffers and Reagents/Sample Buffers &amp; Reagents ->MT::09e4dbf1-40c5-4a92-ace5-3ec31296ac7e##Life Science Research/Products/Protein Sample Preparation/Protein Sample Cleanup/ReadyPrep 2-D Cleanup Kit ->MT::bbf3a0be-188f-45c7-b81e-e9706ec77c02## Life Science Research/Solutions/Technologies/Protein Electrophoresis ->MTS::LUSOVO47B##Life Science Research/Solutions/Technologies/2-D Electrophoresis ->MTS::LUSQG6LPT##Life Science Research/Solutions/Technologies/Western Blotting Life Science Research/Solutions/Applications/Protein Separation and Analysis ->MTS::LUSOU3F7Q##Life Science Research/Solutions/Applications/2-D Electrophoresis and Analysis ->MTS::LUSQF04EH##Life Science Research/Solutions/Applications/Automated Electrophoresis and Analysis ->MTS::LUSQXB15##Life Science Research/Solutions/Technologies/Automated Electrophoresis System -Experion- ->MTS::LUSR04KG4## ->MTS::LUSPPAKG4## Life Science Research/Solutions/Technologies/2-D Electrophoresis/Protein Sample Preparation for 2-D Electrophoresis ->MTS::LUSQH6HYP## Karen Moss Protein Solubilization 02/01/18 08:12 PM 02/01/28 09:43 AM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE LSR /LSR/Technologies/Protein_Electrophoresis N 0 Introduction to Protein Sample Preparation /en-ca/applications-technologies/applications-technologies/protein-solubilization?ID=LUSP108WI

Protein solubilization is the process of breaking interactions involved in protein aggregation, which include disulfide bonds, hydrogen bonds, van der Waals forces, ionic interactions, and hydrophobic interactions. If these interactions are not prevented, proteins can aggregate or precipitate, resulting in artifacts or sample loss. For successful electrophoretic separation, proteins must be well solubilized. This section describes various agents used for protein solubilization, their method of action, and their compatibility to different types of samples. It also provides troubleshooting tips.

Cell lysis and protein solubilization are ideally carried out in the sample buffer that is recommended for the particular electrophoresis technique, especially when native electrophoresis is the method of choice. If this is not possible, proteins must be prepared in sample solubilization solutions that typically contain a number of compounds, including chaotropic agents, detergents, reducing agents, buffers, salts, and ampholytes. These are chosen from a list of compounds that meet the requirements, both electrically and chemically, for compatibility with the electrophoretic technique being used. In these cases, the sample will have to be diluted with concentrated electrophoresis sample buffer to yield a 1x final buffer concentration. Sample solubilization methods for 2-D electrophoresis applications are discussed in the 2-D Electrophoresis section.

Related Sections: Cell Disruption, Removal of Interfering Substances, and Sample Quantitation.

 

Detergents

Detergents are classified as nonionic, zwitterionic, anionic, and cationic, and they disrupt hydrophobic interactions between and within proteins. Some proteins, especially membrane proteins, require detergents for solubilization during isolation as well as to maintain solubility. Nonionic detergents such as NP-40 and Triton X-100 are not very effective in solubilizing hydrophobic proteins; zwitterionic detergents such as CHAPS and sulfobetaines (for example, SB 3–10 or ASB 14) provide higher solubilization efficiency, especially for integral membrane proteins (Luche et al, 2003). Sample preparation for SDS-PAGE commonly uses the anionic detergent sodium dodecyl sulfate (SDS), which is unparalleled in its ability to efficiently and rapidly solubilize proteins.

 

Reducing Agents

Thiol reducing agents such as 2-mercaptoethanol βME and dithiothreitol (DTT) disrupt intramolecular and intermolecular disulfide bonds and are used to achieve complete protein unfolding and to maintain proteins in their fully reduced states (see figure below).

βME is volatile, evaporates from solutions, and reduces protein disulfide bonds by disulfide exchange. There is equilibrium between free thiols and disulfides, so βME is used in large excess in sample buffers to drive the equilibrium reaction toward completion. If the concentration of βME drops and proteins reoxidize, they may yield fuzzy or spurious artifactual bands upon gel electrophoresis of the sample.

DTT is less volatile and is altered during the disulfide reduction reaction to form a ring structure form its original straight chain. The equilibrium favors protein reduction, so lower concentrations of DTT are needed (higher concentrations are recommended for proteins with large numbers of disulfide bonds).

Phosphines such as tributylphosphine (TBP) and triscarboxyethylphosphine (TCEP)* offer an alternative to thiols as reducing agents because they can be used at lower concentration and over a wider pH range than the sulfhydryl reductants.

Reduction of proteins with DTT.

*TCEP is included in Bio-Rad’s XT sample buffers. Though TCEP can be added to SDS-PAGE sample buffer, it must first be neutralized with NaOH; otherwise it will hydrolyze proteins.

 

Chaotropic Agents

Chaotropic compounds such as urea disrupt hydrogen bonds and hydrophobic interactions both between and within proteins. When used at high concentrations, they destroy secondary protein structure and bring otherwise-insoluable proteins into solution.

Urea and substituted urea such as thiourea improve solubilization of hydrophobic proteins.

Samples containing urea and thiourea can be used in SDS-PAGE when diluted with SDS-PAGE sample buffer. In this case, the protein solution may not be heated above 37°C, since urea and thiourea can hydrolyze to cyanate and thiocyanate, respectively, and modify amino groups on proteins (carbamylation), giving rise to artifactual charge heterogeneity.

 

Buffers and Salts

Both pH and ionic strength influence protein solubility; therefore, buffer choice is important, especially when native electrophoresis conditions are required. Many proteins are more soluble at higher pH, so Tris base is often included to elevate the pH. However, proteins differ in their solubility at different pH values, so the use of different buffers can extract different sets of proteins. The choice of buffer and pH of the sample preparation solution can strongly influence which proteins are successfully solubilized.

Even in the presence of detergents, some proteins have stringent salt requirements to maintain their solubility, but salt should be present only if it is an absolute requirement. Excess salt in SDS-PAGE samples causes fuzzy bands and narrowing of gel lanes toward the bottom of the gel. If the ionic strength is very high, no visible bands will appear in the lower part of the gel (a vertical streak will appear instead) and the dye front will be wavy instead of straight.

 

Ideally, cell lysis and protein solubilization are carried out in the sample buffer that is recommended for the particular electrophoresis technique, especially for native electrophoresis. If this is not possible, dilute the protein solution with concentrated electrophoresis sample buffer to yield a 1x final buffer concentration.

ReadyPrep™ protein extraction kits

Bio-Rad offers a number of ready-to-use kits and solutions for protien extraction. These kits and reagents facilitate the solubilization of the sample and preparation for 2D electrophoresis separation:

 

Troubleshooting

Problem Cause Solution
Laemmli sample buffer turns yellow Sample buffer is too acidic Add Tris base until buffer turns
blue again
Sample very viscous High DNA or carbohydrate content

Fragment DNA with ultrasonic waves during cell lysis and protein solubilization

Add endonucleases like Benzonase

Precipitate protein with TCA/acetone (ReadyPrep 2-D cleanup kit) to diminish carbohydrate content

 

References

Luche S et al. (2003). Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis. Proteomics 3, 249–253.

 

Related Content

 
Literature
Number Description Download
3096 Sample Preparation: Tools for Protein Sample Extraction, Cleanup, Fractionation, and Depletion Brochure, Rev B Click to download
6194 Protein Sample Generation General Tips Click to download
6195 Protein Sample Preparation (Human Tissue) Click to download
6196 Protein Sample Preparation (Mammalian Tissue) Click to download
6197 Protein Sample Preparation (Plant Leaves) Click to download
6198 Protein Sample Preparation (Microbial Cultures) Click to download
6199 Buffer Formulations Click to download
 
 
LUSP3KFD4 [x-forwarded-proto] = [http] [x-forwarded-port] = [80] [x-forwarded-for] = [54.224.56.126, 10.232.19.174] [accept] = [text/html,application/xhtml+xml,application/xml;q=0.9,*/*;q=0.8] [seourl] = [/en-ca/applications-technologies/protein-solubilization] [x-amzn-trace-id] = [Root=1-5bc57279-162b964ceafaa04cf32f5cfc] [x-forwarded-server] = [lsds-prod-s.br.aws-livesite.io] [x-forwarded-host] = [www.bio-rad.com] [x-query-string] = [ID=LUSP3KFD4] [host] = [10.232.0.21:1776] [x-request-uri] = [/en-ca/applications-technologies/protein-solubilization] [connection] = [Keep-Alive] [accept-encoding] = [gzip] [user-agent] = [CCBot/2.0 (https://commoncrawl.org/faq/)] AppTech/AppTechDetails pageStyleKey internet/solutions_sub applications-technologies/protein-solubilization LSR LUSP3KFD4 Protein Solubilization Protein Solubilization /webroot/web/html/lsr/solutions/technologies/protein_electrophoresis <p>Protein solubilization is the process of breaking interactions involved in protein aggregation, which include disulfide bonds, hydrogen bonds, van der Waals forces, ionic interactions, and hydrophobic interactions. If these interactions are not prevented, proteins can aggregate or precipitate, resulting in artifacts or sample loss. For successful electrophoretic separation, proteins must be well solubilized. This section describes various agents used for protein solubilization, their method of action, and their compatibility to different types of samples. It also provides troubleshooting tips.</p> <p>Cell lysis and protein solubilization are ideally carried out in the sample buffer that is recommended for the particular electrophoresis technique, especially when native electrophoresis is the method of choice. If this is not possible, proteins must be prepared in sample solubilization solutions that typically contain a number of compounds, including chaotropic agents, detergents, reducing agents, buffers, salts, and ampholytes. These are chosen from a list of compounds that meet the requirements, both electrically and chemically, for compatibility with the electrophoretic technique being used. In these cases, the sample will have to be diluted with concentrated electrophoresis sample buffer to yield a 1x final buffer concentration. Sample solubilization methods for 2-D electrophoresis applications are discussed in the <a href="/en-us/applications-technologies/2-d-electrophoresis?ID=LUSQF04EH">2-D Electrophoresis</a> section.</p> <p><strong>Related Sections</strong>: <a href="/en-us/applications-technologies/cell-disruption?ID=LUSP2D1FX">Cell Disruption</a>, <a href="/en-us/applications-technologies/removal-interfering-substances?ID=LUSP4LJSZ">Removal of Interfering Substances</a>, and <a href="/en-us/applications-technologies/protein-electrophoresis-sample-preparation/sample-quantitation?ID=LUSP5JKAJ">Sample Quantitation</a>.</p> Detergents <p>Detergents are classified as nonionic, zwitterionic, anionic, and cationic, and they disrupt hydrophobic interactions between and within proteins. Some proteins, especially membrane proteins, require detergents for solubilization during isolation as well as to maintain solubility. Nonionic detergents such as NP-40 and Triton X-100 are not very effective in solubilizing hydrophobic proteins; zwitterionic detergents such as CHAPS and sulfobetaines (for example, SB 3&ndash;10 or ASB 14) provide higher solubilization efficiency, especially for integral membrane proteins (Luche et al, 2003). Sample preparation for SDS-PAGE commonly uses the anionic detergent sodium dodecyl sulfate (SDS), which is unparalleled in its ability to efficiently and rapidly solubilize proteins.</p> <div class="top"><a href="#helptop">Back to Top</a></div> Reducing Agents <p>Thiol reducing agents such as 2-mercaptoethanol &beta;ME and dithiothreitol (DTT) disrupt intramolecular and intermolecular disulfide bonds and are used to achieve complete protein unfolding and to maintain proteins in their fully reduced states (see figure below).</p> <p>&beta;ME is volatile, evaporates from solutions, and reduces protein disulfide bonds by disulfide exchange. There is equilibrium between free thiols and disulfides, so &beta;ME is used in large excess in sample buffers to drive the equilibrium reaction toward completion. If the concentration of &beta;ME drops and proteins reoxidize, they may yield fuzzy or spurious artifactual bands upon gel electrophoresis of the sample.</p> <p>DTT is less volatile and is altered during the disulfide reduction reaction to form a ring structure form its original straight chain. The equilibrium favors protein reduction, so lower concentrations of DTT are needed (higher concentrations are recommended for proteins with large numbers of disulfide bonds).</p> <p>Phosphines such as tributylphosphine (TBP) and triscarboxyethylphosphine (TCEP)* offer an alternative to thiols as reducing agents because they can be used at lower concentration and over a wider pH range than the sulfhydryl reductants.</p> <p><img src="/webroot/web/images/lsr/solutions/technologies/protein_electrophoresis_blotting_and_imaging/protein_electrophoresis/technology_detail/pet132_img1.jpg" alt="" width="245" height="264" /></p> <p class="caption"><strong>Reduction of proteins with DTT.</strong></p> <p><em>*TCEP is included in Bio-Rad&rsquo;s XT sample buffers. Though TCEP can be added to SDS-PAGE sample buffer, it must first be neutralized with NaOH; otherwise it will hydrolyze proteins.</em></p> <div class="top"><a href="#helptop">Back to Top</a></div> Chaotropic Agents <p>Chaotropic compounds such as urea disrupt hydrogen bonds and hydrophobic interactions both between and within proteins. When used at high concentrations, they destroy secondary protein structure and bring otherwise-insoluable proteins into solution.</p> <p>Urea and substituted urea such as thiourea improve solubilization of hydrophobic proteins.</p> <p>Samples containing urea and thiourea can be used in SDS-PAGE when diluted with SDS-PAGE sample buffer. In this case, the protein solution may not be heated above 37&deg;C, since urea and thiourea can hydrolyze to cyanate and thiocyanate, respectively, and modify amino groups on proteins (carbamylation), giving rise to artifactual charge heterogeneity.</p> <div class="top"><a href="#helptop">Back to Top</a></div> Buffers and Salts <p>Both pH and ionic strength influence protein solubility; therefore, buffer choice is important, especially when native electrophoresis conditions are required. Many proteins are more soluble at higher pH, so Tris base is often included to elevate the pH. However, proteins differ in their solubility at different pH values, so the use of different buffers can extract different sets of proteins. The choice of buffer and pH of the sample preparation solution can strongly influence which proteins are successfully solubilized.</p> <p>Even in the presence of detergents, some proteins have stringent salt requirements to maintain their solubility, but salt should be present only if it is an absolute requirement. Excess salt in SDS-PAGE samples causes fuzzy bands and narrowing of gel lanes toward the bottom of the gel. If the ionic strength is very high, no visible bands will appear in the lower part of the gel (a vertical streak will appear instead) and the dye front will be wavy instead of straight.</p> <div class="top"><a href="#helptop">Back to Top</a></div> <p>Ideally, cell lysis and protein solubilization are carried out in the sample buffer that is recommended for the particular electrophoresis technique, especially for native electrophoresis. If this is not possible, dilute the protein solution with concentrated electrophoresis sample buffer to yield a 1x final buffer concentration.</p> <p><strong>ReadyPrep&trade; protein extraction kits</strong></p> <p>Bio-Rad offers a number of ready-to-use kits and solutions for protien extraction. These kits and reagents facilitate the solubilization of the sample and preparation for 2D electrophoresis separation:</p> <ul> <li><a href="/en-us/product/readyprep-protein-extraction-kit-total-protein?ID=749eaa83-8fb4-4854-bc8a-84ab01195fb9">ReadyPrep extraction kit (total protein)</a></li> <li><a href="/en-us/sku/1632102-readyprep-sequential-extraction-kit-reagent-1?ID=1632102">ReadyPrep sequential protein extraction reagent</a></li> <li><a href="/en-us/sku/1632085-readyprep-protein-extraction-kit-soluble-insoluble">ReadyPrep sequential protein extraction (soluble/insoluble)</a></li> </ul> <div class="top"><a href="#helptop">Back to Top</a></div> Troubleshooting <table class="pd_table pd_gridlines" border="0"> <tbody> <tr class="pd_tableheader pd"> <td width="26%"><strong>Problem</strong></td> <td width="26%"><strong>Cause</strong></td> <td><strong>Solution</strong></td> </tr> <tr> <td valign="top">Laemmli sample buffer turns yellow</td> <td valign="top">Sample buffer is too acidic</td> <td valign="top">Add Tris base until buffer turns<br /> blue again</td> </tr> <tr> <td valign="top">Sample very viscous</td> <td valign="top">High DNA or carbohydrate content</td> <td valign="top"> <p>Fragment DNA with ultrasonic waves during cell lysis and protein solubilization</p> <p>Add endonucleases like Benzonase</p> <p>Precipitate protein with TCA/acetone (<a href="/en-us/product/readyprep-2-d-cleanup-kit?ID=bbf3a0be-188f-45c7-b81e-e9706ec77c02">ReadyPrep 2-D cleanup kit</a>) to diminish carbohydrate content</p> </td> </tr> </tbody> </table> <div class="top"><a href="#helptop">Back to Top</a></div> References <p>Luche S et al. (2003). Evaluation of nonionic and zwitterionic detergents as membrane protein solubilizers in two-dimensional electrophoresis. Proteomics 3, 249&ndash;253.</p> <div class="top"><a href="#helptop">Back to Top</a></div> Protocols <table id="carttablealigned" class="literature_table" style="height: auto; width: 583px;" border="0" cellspacing="0" cellpadding="0"> <tbody> <tr></tr> <tr> <td width="100">6194</td> <td width="350">Protein Sample Generation General Tips</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6194.pdf" target="_blank" rel="noopener noreferrer"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6195</td> <td width="350">Protein Sample Preparation (Human Tissue)</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6195.pdf" target="_blank" rel="noopener noreferrer"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6196</td> <td width="350">Protein Sample Preparation (Mammalian Tissue)</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6196.pdf" target="_blank" rel="noopener noreferrer"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6197</td> <td width="350">Protein Sample Preparation (Plant Leaves)</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6197.pdf" target="_blank" rel="noopener noreferrer"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6198</td> <td width="350">Protein Sample Preparation (Microbial Cultures)</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6198.pdf" target="_blank" rel="noopener noreferrer"><span>Click to download</span></a></td> </tr> <tr> <td width="100">6199</td> <td width="350">Buffer Formulations</td> <td class="pdf"><a class="pdf" href="/webroot/web/pdf/lsr/literature/Bulletin_6199.pdf" target="_blank" rel="noopener noreferrer"><span>Click to download</span></a></td> </tr> </tbody> </table> 3096 templatedata/internet/documentation/data/LSR/Literature/3096_0910140925966.xml Life Science Research/Products/Protein Sample Preparation/Protein Extraction/MicroRotofor Cell Lysis Kits ->MT::5e97c5be-3707-4c08-ae55-25e883f2ab99##Life Science Research/Products/Protein Sample Preparation/Protein Extraction/ReadyPrep Protein Extraction Kit (Total Protein) ->MT::749eaa83-8fb4-4854-bc8a-84ab01195fb9##Life Science Research/Products/Sample Quantitation/Protein Assay Kits and Cuvettes/<em>RC DC</em> Protein Assay ->MT::f58fdf1d-5d0b-4f31-93e2-f812bd7e7eab##Life Science Research/Products/Electrophoresis and Blotting/Protein Electrophoresis and Blotting/Electrophoresis Buffers and Reagents/Sample Buffers &amp; Reagents ->MT::09e4dbf1-40c5-4a92-ace5-3ec31296ac7e##Life Science Research/Products/Protein Sample Preparation/Protein Sample Cleanup/ReadyPrep 2-D Cleanup Kit ->MT::bbf3a0be-188f-45c7-b81e-e9706ec77c02## Life Science Research/Solutions/Technologies/Protein Electrophoresis ->MTS::LUSOVO47B##Life Science Research/Solutions/Technologies/2-D Electrophoresis ->MTS::LUSQG6LPT##Life Science Research/Solutions/Technologies/Western Blotting Life Science Research/Solutions/Applications/Protein Separation and Analysis ->MTS::LUSOU3F7Q##Life Science Research/Solutions/Applications/2-D Electrophoresis and Analysis ->MTS::LUSQF04EH##Life Science Research/Solutions/Applications/Automated Electrophoresis and Analysis ->MTS::LUSQXB15##Life Science Research/Solutions/Technologies/Automated Electrophoresis System -Experion- ->MTS::LUSR04KG4## ->MTS::LUSPPAKG4## Life Science Research/Solutions/Technologies/2-D Electrophoresis/Protein Sample Preparation for 2-D Electrophoresis ->MTS::LUSQH6HYP## Karen Moss Protein Solubilization 02/01/18 08:12 PM 02/01/28 09:43 AM AR,BO,BR,CA,CL,CO,CR,DO,EC,SV,GU,GT,HT,HN,JM,MX,NI,PA,PY,PE,PR,TT,US,UY,VE,AU,BD,BN,KH,CN,HK,IN,ID,JP,KR,MO,MY,FM,NP,NZ,PW,PG,PH,SG,SB,LK,TW,TH,TO,VU,AL,DZ,AI,AM,AT,AZ,BH,BE,BA,BW,BG,BF,CM,HR,CY,CZ,DK,EG,ER,EE,ET,FO,FI,FR,GF,PF,GA,GE,DE,GH,GR,GP,HU,IS,IE,IL,IT,JO,KZ,KE,XK,KW,LV,LB,LI,LT,LU,MK,MG,ML,MT,MQ,MU,MD,MS,MA,NL,NG,NO,OM,PK,PS,PL,PT,QA,RO,RU,ST,SA,SN,RS,SK,SI,ZA,ES,SE,CH,TZ,TG,TN,TR,UG,UA,AE,UK,UZ,VA,EH,YE LSR /LSR/Technologies/Protein_Electrophoresis N 0
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