Total protein detection and immunodetection can be performed using colorimetric, chemiluminescence, and fluorescence development and imaging techniques. Once proteins have been transferred to a membrane, they can be visualized using a variety of specialized detection reagents. Total protein detection allows visualization of all the proteins on the blot while immunological detection (immunodetection) methods employ antibody or ligand conjugates for visualization of specific proteins of interest. This section reviews the various total protein and immunological detection methods available.
Related Topics: Protein Blotting Methods, Membranes and Blotting Papers, Transfer Buffers, and Transfer Conditions.
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Protein detection systems and their sensitivity.
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