Protein Blotting Detection and Imaging

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Overview

Total protein detection and immunodetection can be performed using colorimetric, chemiluminescence, and fluorescence development and imaging techniques. Once proteins have been transferred to a membrane, they can be visualized using a variety of specialized detection reagents. Total protein detection allows visualization of all the proteins on the blot while immunological detection (immunodetection) methods employ antibody or ligand conjugates for visualization of specific proteins of interest. This section reviews the various total protein and immunological detection methods available.

Related Topics: Protein Blotting Methods, Membranes and Blotting Papers, Transfer Buffers, and Transfer Conditions.

Protein Detection Systems

Protein Detection Systems

Protein detection systems and their sensitivity.

Related Content

Literature
Number Description Download
2032 Western Blotting Detection Reagents Brochure, Rev F Click to download
2895 Protein Blotting Guide, Interactive PDF, Rev B Click to download
Number Description Options
6216 Detection Buffer Formulations Click to download
6217 Total Protein Detection Click to download
6218 Blot Stripping & Reprobing Click to download
6219 Immunodetection Click to download
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