In conventional PCR, problems with reaction components and amplification protocols are diagnosed by running a gel. If you experience any of the symptoms pictured below when visualizing PCR products by agarose gel electrophoresis, click on the corresponding photo to learn about possible causes and treatments.
Related Topics: PCR Instruments, PCR Reagents, PCR Assay Design and Optimization, and PCR Analysis.
Click on the image below that is most representative of your own gel to find out the probable cause and specific solutions to address your problem.
No Band or Faint Band
Nonspecific Bands or Primer-Dimers
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