The Bio-Plex Suspension array system utilizes xMAP technology licensed from Luminex to permit the multiplexing of up to 100 different assays within a single sample. This technique involves 100 distinctly colored bead sets created by the use of two fluorescent dyes at various ratios. In a sandwich immunoassay, one antibody to a specific analyte is attached to a set of beads with the same color, and the second antibody against the analyte is attached to a fluorescent dye. A dual detection flow cytometer is used to identify the different assays based on bead color in one channel, and to quantify the analyte by measurement of the reporter dye fluorescence in another channel.
In a typical Bio-Plex assay, a capture molecule conjugated to a color-coded bead binds to a target analyte (1) followed by binding with biotinylated detection anitbody (2) and a reporter molecule, streptavidin-PE (3).
In the Bio-Plex array reader, a red classification and a green reporter laser illuminate individual beads to identify each bead's spectral address and associated reporter signal.
Related Topics: Bio-Plex Assays and Bio-Plex Assay Data Analysis.
After the analytes have been captured and coupled with the detection antibodies, the Bio-Plex instrument or array reader is then used to detect the identity and determine the quantity of the analytes.
Components of the Bio-Plex reader. APD, avalanche photodiode detector; DD, doublet discriminator channel, discriminates single beads from aggregated beads; CL1, classify channel, allows multiplexing, detects dye inside beads; and RP1, reporter channel, quantitates assay in this channel.
The Bio-Plex array reader comprises four different components:
Dr Spinale, Professor of Cardiothoracic Surgery at Medical University of South Carolina, explains how the Bio-Plex system allowed him to tackle important questions in childhood disease research.
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