Image Analysis Software

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Overview

A robust imaging software package is required to analyze data and draw conclusions from PAGE applications. Sophisticated gel analysis software provides a variety of tools that enhance the user's ability to evaluate the acquired data. This section provides an overview of the various gel analysis software options offered by Bio-Rad.

Related Topics: Imaging Systems.

Analysis Software

The software magnifies, rotates, resizes, adjusts contrast and brightness of, and annotates gel images, which can then be printed using standard and thermal printers. All data in the images can be quickly and accurately quantified. The software can measure average and total quantities and determine relative and actual amounts of proteins. Gel imaging software is also capable of determining the presence or absence and up or down regulation of proteins, their molecular weights, isoelectric points, and other values.

Bio-Rad offers three different software packages for gel imaging and analysis. Refer to the table below to find out the suitability of each of these software packages for your specific application:

  • Image Lab™ Software — image acquisition and analysis software that runs the ChemiDoc™ MP, ChemiDoc™ Touch, ChemiDoc™ XRS+, Gel Doc™ XR+, and Gel Doc™ EZ Imaging Systems. The software allows automatic configuration of these imaging systems with appropriate filters and illumination sources and allows manual or automated analysis of PAGE gels and western blots. Image Lab Software automatically indentifies the image with the best signal-to-noise ratio and generates a report, and it provides sophisticated algorithms to determine the number of lanes and bands in the image. It can quantitate and compare signal intensities to determine relative signal and generate other data such as relative front values for molecular weight determinations

     
  • Quantity One® 1-D Analysis Software — acquires, quantitates, and analyzes a variety of data from radioactive, chemiluminescent, fluorescent, and color-stained samples acquired from densitometers, storage phosphor imagers, fluorescence imagers, and gel documentation systems. The software allows automatic configuration of these imaging systems with appropriate filters, lasers, LEDs, and other illumination sources, and it allows manual or automated analysis of PAGE gels and western blots

     
  • PDQuest™ 2-D Analysis Software — used for 2-D gel electrophoretic analysis. The software is a popular tool for digitized gel image analysis, which includes spot detection and quantitation, gel comparison, and statistical analysis. PDQuest Software seamlessly integrates with any of Bio-Rad's image acquisition instruments, and can also control the EXQuest™ Spot Cutter. Its advanced annotation feature can be used to label spots with text, URL links, document links, or mass spectrometry data

Imaging Software Application Guide

  Gel Analysis
  Image Lab Quantity One PDQuest
Image acquisition  
Automated analysis
Automated imaging and analysis    
1-D gel analysis  
Colony counting    
Dot/slot blot analysis  
2-D gel analysis    
Image stacking*    
Integrated gel excision (spot cutting)  
Integrated relational database      
1-D gel cluster analysis and dendrogram tools      
Phenotype analysis      
Fatty acid profile analysis      
Enzyme activity panel analysis      
DNA and protein sequence data analysis      
Microarray and gene chip data analysis      
Data integration      
U.S. FDA 21 CFR Part 11 compliance tools
 

* Image stacking is available in PDQuest Advanced Software only.

Related Content

Using Image Lab™ Software with the ChemiDoc™ MP, ChemiDoc Touch, ChemiDoc XRS+, Gel Doc™ XR+, and Gel Doc EZ Systems
This tutorial provides an overview of image acquisition and analysis, including basic and advanced features.
 
Installing the Molecular Imager® Gel Doc™ XR+ System
This tutorial guides you through installing the Gel Doc XR+ system and provides troubleshooting techniques for common problems. Information is provided for new installations on both PC and Macintosh systems
 
Gel Doc EZ System Tour
In this interactive demonstration you will be able to test drive the Gel Doc EZ imager and dig deaper into the instrument's capabilities, specifications and features.
 
Quantitative PCR (qPCR): Hallmarks of an Optimized SYBR® Green I Assay
This tutorial describes the characteristics of an optimized SYBR® Green I quantitative PCR (qPCR) assay. qPCR assays must be optimized to ensure results that are biologically and statistically significant. Topics include a brief review of qPCR chemistry, with an emphasis on SYBR® Green I reactions, and definitions of the four main characteristics, or hallmarks, of an optimized qPCR assay.
 
Literature
Number Description Download
5888 Bio-Rad Imaging Systems Family Brochure, Rev E Click to download
5989 Imaging Fluorescently Stained Gels with Image Lab Software Quick Start Guide, Rev A Click to download
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