Fluorescent Assay Kits

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Fluorescent Assay Kits

Overview

Filter Selection Guide
Application Excitation Filter Catalog # Emission Filter Catalog #
DNA quantitation (Hoechst 33258) EX 360/40 (340–380 nm) 170-2420 EM 460/10 (455–465 nm) 170-2421
β-Galactosidase (MUG) EX 360/40 (340–380 nm) 170-2420 EM 460/10 (455–465 nm) 170-2421
β-Glucuronidase (MUGluc) EX 360/40 (340–380 nm) 170-2420 EM 460/10 (455–465 nm) 170-2421
DNA quantitation (PicoGreen) EX 480/20 (470–490 nm) 170-2427 EM 520/10 (515–525 nm) 170-2424
RNA quantitation (RiboGreen) EX 490/10 (485–495 nm) 170-2422 EM 520/10 (515–525 nm) 170-2424
Oligonucleotide/protein quantitation (OliGreen) EX 480/20 (470–490 nm) 170-2427 EM 520/10 (515–525 nm) 170-2424
Protein quantitation (NanoOrange) EX 480/20 (470–490 nm) 170-2427 EM 590/10 (585–595 nm) 170-2425
Fluorescein EX 490/10 (485–495 nm) 170-2422 EM 520/10 (515–525 nm) 170-2424
Ethidium bromide EX 510/10 (505–515 nm) 170-2423 EM 620/10 (615–625 nm) 170-2426
wtGFP (wild-type Green Fluorescent Protein) EX 390/22 (379–401 nm) 170-2443 EM 510/10 (505–515 nm) 170-2445
EGFP (red-shifted Green Fluorescent Protein) EX 490/10 (485–495 nm) 170-2422 EM 510/10 (505–515 nm) 170-2445
EBFP (blue-emitting Green Fluorescent Protein) EX 380/30 (365–395 nm) 170-2444 EM 440/10 (435–445 nm) 170-2446
GFPuv (UV-excited Green Fluorescent Protein) EX 390/22 (379–401 nm) 170-2443 EM 510/10 (505–515 nm) 170-2445

User-selectable filters support a multitude of applications with a broad range of excitation and emission wavelengths. Custom filter sets are also available from your local Bio-Rad sales representative. This table lists commonly used fluorescent assays and recommended excitation and emission filters.

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DNA Quantitation Kit
You can quickly and accurately quantitate dsDNA in solution, even in the presence of RNA or protein, with Bio-Rad's fluorescent DNA quantitation kit. DNA is quantitatively stained with a bis-benzimide dye (Hoechst 33258), which is excited with 346 nm light and emits blue light at 460 nm that is easily detected using a fluorometer such as the VersaFluor fluorometer. The kit quantitates dsDNA in the range of 10 ng/ml to 5 µg/ml and contains reagents for 750 standard 2 ml reactions

FluorAce β-Galactosidase Reporter Assay Kit
You can detect β-galactosidase activity easily and accurately with the FluorAce β-galactosidase reporter assay kit. Simply add the fluorescent substrate β-4-methylumbelliferyl β-D-galactopyranoside (MUG) to the cell or microorganism medium. The substrate penetrates the cell and is hydrolyzed by β-galactosidase, producing the fluorescent compound 4-methylumbelliferone (4MU), which is easily detected using a fluorometer such as the VersaFluor fluorometer. The kit contains reagents for 200 standard 1.5 ml reactions

FluorAce β-Glucuronidase Reporter Assay Kit
You can fluorescently detect β-glucuronidase activity quickly and precisely with the FluorAce β-glucuronidase reporter assay kit. The substrate 4-methylumbelliferyl β-D-glucuronide (MUGluc) is hydrolyzed by β-glucuronidase, producing a fluorogenic compound that is easily detected using a fluorometer such as the VersaFluor fluorometer. The kit contains reagents for 200 standard 1.5 ml reactions

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FluorAce β-Glucuronidase Reporter Assay Kit

1703151
Fluorescent assay kit contains reaction buffer, MUGIuc substrate, stop buffer, 4MU control, β-glucuronidase; sufficient for 200 standard 1.5 ml reactions

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Number Description Options
4100129 Instruction Manual, FluorAce beta-Glucuronidase Reporter Assay Click to download
4100128 Instruction Manual, FluorAce beta-Galactosidase Reporter Assay Kit, Rev A Click to download
4006108 Instruction Manual, CE dsDNA 1000 Fluorescence Detection and Analysis Kit Click to download

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