Applications & Technologies

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This section offers extensive technical information to guide you through your research and help you overcome obstacles in your experiments. Here you will find workflows, protocols, FAQs, videos and product demonstrations, and a wealth of technical information on the theory and principles behind important and cutting-edge life science technologies.

Therapeutic Discovery & Development

  • Drug Discovery and Development

    Bio-Rad products and services span the entire range of drug discovery and development activities, from target identification to scale-up and manufacturing.

  • Cancer Immunotherapy Development

    Learn about trends in cancer immunotherapy development, such as CAR T and various technology solutions that provide sensitivity, precision, and absolute quantification.

  • Biologic Development

    Biologic development brings patients closer to personalized medicine to treat health problems.

Genomics & Gene Expression

  • PCR (Polymerase Chain Reaction)

    Learn how PCR works, how to choose the right PCR instrument, how to design, optimize, analyze, and troubleshoot PCR assays.

  • Real-Time PCR (qPCR)

    Discover an array of solutions to optimize, execute, and troubleshoot MIQE-compliant real-time PCR experiments.

  • Introduction to Digital PCR

    Digital PCR is a breakthrough technology that provides ultrasensitive and absolute nucleic acid quantification.

  • Genomics

    Explore different aspects of genomics — from functional genomics and biomarker discovery to mutation analysis, gene expression, transfection, and epigenetics.

  • Targeted Genome Editing

    Learn about the major systems for targeted genome editing, including CRISPR/Cas9, some key applications, and workflow options.

  • Horizontal Electrophoresis

    Gel boxes, running buffers, available agarose types, as well as factors affecting resolution of DNA fragments, to help with agarose gel electrophoresis of DNA.

  • Pulsed Field Gel Electrophoresis

    How can large DNA fragments be separated using PFGE? How to prepare plugs, optimize sample resolution, analyze images, and troubleshoot PFGE gels? Explore.

Cell Biology & Biochemistry

  • Biomarker Analysis

    Details on how biomarkers such as hormones, cytokines, chemokines, growth factors, and phosphoproteins are used to investigate physiological or pharmacological mechanisms.

  • Stem Cell Research

    Tips for isolation, maintenance, differentiation, transfection and analysis of stem cells.

  • Introduction to Transfection

    Information and tips on electroporation, viral transfection vector, lipofection, and biolistics transformation for planning and selecting the appropriate transfection method.

  • Cell Counting Methods

    Manual and automated cell counting methods and their application to gene expression studies are presented and discussed here.

  • Multiplex Immunoassays

    Details on the experimental design, data collection, analysis, and validation of multiplex protein assays using the Bio-Plex suspension array system.

Proteomics & Protein Purification

  • Introduction to Chromatography

    Description of the different types of chromatography methods available and how they can be applied to guide you in your protein purification experiments.

  • Protein Purification and Isolation

    Learn how protein purification by chromatography is used in monoclonal antibody discovery, viral contaminant removal, and preparative protein isolation.

  • Separation, Transfer, and Analysis of Proteins

    Bio-Rad’s V3 Western Workflow facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins.

  • Introduction to Protein Electrophoresis

    Protocols, video tutorials, and selection guides to help you at every step of your electrophoresis experiments.

  • Introduction to Western Blotting

    Learn more about western blotting techniques. Find step-by-step protocols and helpful tips on equipment, membranes, transfer conditions, and detection methods.

  • Fluorescent Western Blotting

    Learn how fluorescence western blotting and total protein normalization can help with quantitative and accurate protein expression analyses.

  • 2-D Electrophoresis

    Useful information from sample preparation to imaging of 2-D gels, along with protocols, video tutorials, demonstrations, and troubleshooting tips.

  • Imaging and Analysis

    Comparison of parameters, sensitivity, and data analysis using Bio-Rad imaging systems and software to determine which imaging system is ideal for your application.

Resources

  • Contact an Expert

    Consult with a specialist about how Bio-Rad can help you with your research problems.

  • Technical Support

    Reach our award-winning technical team by phone or email.


2-D Electrophoresis and Analysis

2-DE is an essential step in biomarker discovery workflow, whether your goal is protein characterization, purification and profiling, or posttranslational modification studies.

Drug Discovery and Development

Bio-Rad products and services span the entire range of drug discovery and development activities, from target identification to scale-up and manufacturing.

Targeted Genome Editing

Learn about the major systems for targeted genome editing, including CRISPR/Cas9, some key applications, and workflow options.

Biologic Development
Biologic development brings patients closer to personalized medicine to treat health problems.
Biomarker Analysis

Details on how biomarkers such as hormones, cytokines, chemokines, growth factors, and phosphoproteins are used to investigate physiological or pharmacological mechanisms.

Genomics

Explore different aspects of genomics — from functional genomics and biomarker discovery to mutation analysis, gene expression, transfection, and epigenetics.

Cancer Immunotherapy Development
Learn about trends in cancer immunotherapy development, such as CAR T and various technology solutions that provide sensitivity, precision, and absolute quantification.
Label-Free Biomolecular Interactions

Various aspects of how to develop, optimize, and validate biomolecular interaction assays using surface plasmon resonance (SPR) technology.

Separation, Transfer, and Analysis of Proteins

Bio-Rad’s V3 Western Workflow™ facilitates speed and validation at each step of a western blotting experiment — from running gels to quantifying proteins.

Stem Cell Research

Tips for isolation, maintenance, differentiation, transfection and analysis of stem cells.

Protein Purification and Isolation

Learn how protein purification by chromatography is used in monoclonal antibody discovery, viral contaminant removal, and preparative protein isolation.

Digital PCR and Real-Time PCR (qPCR) Choices for Different Applications

Find out how quantitative PCR (qPCR) and digital PCR (dPCR) compare for specific applications and when to use one over the other.

Automate Electrophoresis and Analysis

How can automation in electrophoresis enhance reliability in experiments assessing the purity and yield of proteins or evaluating the integrity of RNA?

 

Introduction to Protein Electrophoresis

Protocols, video tutorials, and selection guides to help you at every step of your electrophoresis experiments.

PCR (Polymerase Chain Reaction)
Learn how PCR works, how to choose the right PCR instrument, how to design, optimize, analyze, and troubleshoot PCR assays.
Introduction to Western Blotting
Learn more about western blotting techniques. Find step-by-step protocols and helpful tips on equipment, membranes, transfer conditions, and detection methods.
What is Real-Time PCR (qPCR)?
Discover an array of solutions to optimize, execute, and troubleshoot MIQE-compliant real-time PCR experiments.
Fluorescent Western Blotting

Learn how fluorescence western blotting and total protein normalization can help with quantitative and accurate protein expression analyses.

Imaging and Analysis
Comparison of parameters, sensitivity, and data analysis using Bio-Rad imaging systems and software to determine which imaging system is ideal for your application.
Introduction to Digital PCR

Digital PCR is a breakthrough technology that provides ultrasensitive and absolute nucleic acid quantification.

2-D Electrophoresis

Useful information from sample preparation to imaging of 2-D gels, along with protocols, video tutorials, demonstrations, and troubleshooting tips.

Introduction to Transfection

Information and tips on electroporation, viral transfection vector, lipofection, and biolistics transformation for planning and selecting the appropriate transfection method.

Horizontal Electrophoresis

Gel boxes, running buffers, available agarose types, as well as factors affecting resolution of DNA fragments, to help with agarose gel electrophoresis of DNA.

Cell Counting Methods

Manual and automated cell counting methods and their application to gene expression studies are presented and discussed here.

Pulsed Field Gel Electrophoresis

How can large DNA fragments be separated using PFGE? How to prepare plugs, optimize sample resolution, analyze images, and troubleshoot PFGE gels? Explore.

Multiplex Immunoassays

Details on the experimental design, data collection, analysis, and validation of multiplex protein assays using the Bio-Plex® suspension array system.

Experion™ Automated Electrophoresis System

The microfluidic technology used in Bio-Rad’s Experion™ system helps to automate protein and nucleic acid separation, detection, and analysis.

ProteOn™ XPR36 Surface Plasmon Resonance (SPR) System

How does this label-free system perform analysis of biomolecular interactions? How do you choose the right sensor chip for your analysis? Find out.

Introduction to Chromatography

Description of the different types of chromatography methods available and how they can be applied to guide you in your protein purification experiments.

 
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