Product and Application Tutorials

The following tutorials have been developed by the Bio-Rad Life Science Technical Support Team and are intended to provide assistance with common customer support needs. The tutorials are presented in English unless noted otherwise.

Troubleshooting Well Factor Errors on the iCycler iQ and MyiQ Real-Time PCR Detection Systems (duration 20:13)

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Description:
This tutorial discusses well factor errors on the iCycler iQ and MyiQ real-time PCR detection systems. It provides information about why the software on these systems fails well factor data, and highlights common well factor error messages. Troubleshooting tips and information about available replacement parts are also provided.

2-D Electrophoresis: Avoiding Horizontal Streaks (duration 15:00)

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Description:
Horizontal streaks in 2-D gels are the result of poor protein separation during isoelectric focusing (IEF). This tutorial discusses the most common sources of horizontal streaks in 2-D gels: problems with sample preparation and inappropriate IEF conditions.

Mini-PROTEAN Tetra Cell: Features, Parts, and Assembly (duration 11:30)

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Description:
This tutorial highlights the main features and components of the Mini-PROTEAN Tetra cell and shows you how to assemble the unit and its handcasting accessories. Information about the compatibility of its components with the Mini-PROTEAN 3 and Mini Trans-Blot cells is also provided.

Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell: Application, Assembly, and Troubleshooting (duration 11:00)

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Description:
This presentation highlights the main features of the Trans-Blot semi-dry SD transfer cell, shows you how to assemble the unit, and provides information about available replacement parts. Important troubleshooting tips are also provided.

Data Analysis: Real-Time Quantitative PCR (duration 28:10)

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Description:
This tutorial pertains to the analysis of real-time quantitative PCR (qPCR) data using both absolute and relative quantification methods. The methods presented here can be applied to both Bio-Rad and MJ real-time systems.

DNA Engine Opticon 2 Real-Time PCR System: Installation Overview (duration 21:38)

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Description:
This tutorial shows the basic steps involved in reinstalling or upgrading a DNA Engine Opticon 2 system. This procedure is useful in situations where any of the following conditions arise:  computer crashed, hard drive failed, instrument is moved, computer is upgraded.

Experion Software Version 2.1: Using the New Compare Run Feature (duration 4:41)

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Description:
This tutorial shows how to use the new Compare Run feature of Experion software version 2.1. This feature allows side-by-side comparison of different runs.

CHT Ceramic Hydroxyapatite Packing Tutorial: Packing CHT in the Bio-Rad InPlace column (duration 8:11)

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Description:
Bio-Rad offers optimized protocols and hardware for packing and unpacking CHT. The guidelines for handling and packing are recommended for all CHT users to ensure reproducible, high-quality results and to extend the life of the CHT media.

CHT Ceramic Hydroxyapatite Packing Tutorial : Packing CHT in an Open Process Column (duration 6:59)

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Description:
Ceramic hydroxyapatite is widely used in open process chromatography columns. Open columns typically have a fixed bottom endpiece with an adjustable upper piston. These columns are simple in design and allow for easy column packing, unpacking, operation, and cleaning. However, special considerations and guidelines are required when using CHT with open columns to ensure optimal packing and purification.